PROTEOMICS ANALYSIS

Two-dimensional gel electrophoresis

• Sample preparation
• 2D-PAGE first and second dimensions

Protein detection techniques

• Organic dyes, silver staining, fluorescence-based DIGE

Image analysis

• Computerised quantitative image analysis

Protein identification via mass spectrometry

• Basic principles and most commonly used tools
• Ion sources
• Mass-selective analysers

Applied aspects of MALDI-TOF analysis in proteomics

• Sample preparation and matrixPeptide mass fingerprinting

References