Proteomics

Learn about the ins and outs of proteomics, one of the state of the art techniques used in nutrigenomics research. The proteome is, in analogy with the genome, defined as the complete set of proteins present in a cell or tissue at a given time. Study of the proteome of different samples in nutrition research, will provide valuable insights into the interaction between nutrition and a cell, tissue or whole organism. 
In this highly interactive module you take the role of a PhD student who just arrived in a proteomics lab. You will design, virtually perform and analyze a genuine nutrigenomics experiment. By doing quizzes and exercises you will get an in-depth view of proteomics. You will learn the theoretical principles behind the experiments, for better judgment of (published) proteomics results and for designing your own proteomics experiments. In this virtual practical you will use 2D gel electrophoresis followed by MALDI-TOF mass spectrometry to identify your proteins of interest. You will be introduced to web-tools and -resources to analyze proteomics data and to find meaningful answers to your research questions. In addition, different strategies and experimental designs will be highlighted.
 

Keywords/Key phrases

Proteomics, protein, 2D-gel electrophoresis, MALDI-TOF, mass spectrometry, nutrigenomics